Solution reagent HOT FIREPol® GC Master Mix

DNA polymeraseagarose gelTaq DNA polymerase

Excellent amplification with templates up to 79% GC content. Suitable for templates up to 5 kb. Suitable for fragment analysis. Description HOT FIREPol® GC Master Mix has been developed for working with difficult GC-rich templates and DNA secondary structures. It shows excellent amplification with templates up to 79% GC content. The master mix contains hot-start Taq polymerase HOT FIREPol®, MgCl2, dNTPs, and a special buffer for high-yield GC-rich amplification. Extra vials of 100% DMSO and 25 mM MgCl2 enable flexibility in reaction optimization. Reagents Provided HOT FIREPol® GC Master Mix - 1 ml | 250 rxn 25 mM MgCl2 - 0.5 ml DMSO 100% - 0.5 ml Properties Concentration: 5x Hot-start: yes, initial activation in 12-15 min. Ready to load: no Fidelity: 1 x Taq Cloning Type: T/A cloning Amplicon Size: up to 5 kb Difficult templates: robust on GC-rich templates Mix Composition: HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabling hot-start. 5x HOT FIREPol® GC Buffer with 7.5 mM MgCl2: 1x PCR solution –1.5 mM MgCl2. dNTPs: dATP, dCTP, dGTP and dTTP. Bovine serum albumin (BSA) to enhance PCR reaction. In separate vials: 100% DMSO is included in the kit in a separate vial. DMSO is recommended as a PCR additive for templates with high GC content. In some cases, DMSO is also required to relax secondary structures. 25 mM MgCl2 for further optimization. Some applications of this product may require a license which is not provided by the purchase of this product.