Solution reagent kit Solis Hot Start HiFi Master Mix

for PCRhot start

User-friendly, versatile high-fidelity Master Mix for precise and robust PCR - High-fidelity amplification -> 100x higher than Taq - Superior performance with a variety of amplicons (between 27-79%) - Long range PCR – up to 22 kb DNA, depending on the target - Broad multiplexing capabilities - Benchtop stability up to 24 hours - Efficient dUTP tolerance – great for bisulfite sequencing workflows - Inherently stable due to proprietary Stability TAG technology - Tolerant to common PCR inhibitors Description Solis Hot Start HiFi Master Mix is a 5x-concentrated, ready-to-use solution designed for precise singleplex and multiplex PCR applications. It features Solis Hot Start HiFi DNA Polymerase with 3′→5′ exonuclease (proofreading) activity, offering over 100x higher fidelity compared to wild-type Taq polymerase, as demonstrated through single-molecule real-time (SMRT) sequencing. The polymerase in the master mix is chemically modified to enable hot-start PCR, enhancing specificity and accuracy, minimizing mispriming and extension from non-specifically annealed primers and primer-dimers. This makes the mix suitable for applications that demand high fidelity PCR, such as sequencing, cloning, and long-range PCR – supporting amplification of fragments up to 22 kb DNA depending on the target. With improved tolerance for both GC- and AT-rich regions (27–79% GC content), the mix ensures reliable amplification of challenging targets. Its superior multiplexing performance enables even amplification of diverse amplicons, making it ideal for complex assay designs. The DNA polymerase included in Solis Hot Start HiFi Master Mix has efficient toleration of dUTPs, making it well suited for amplification of bisulfite treated DNA (e.g., for bisulfite sequencing), damaged DNA (e.g., FFPE samples). Robust performance is further supported by demonstrated inhibitor tolerance, ensuring consistent and reliable results even in the presence of common PCR inhibitors. Additionally, the Solis Hot Start HiFi Master Mix is room temperature stable for up to 1 month due to the use of Stability TAG – Solis BioDyne’s proprietary and patented polypeptide stabilization technology that is incorporated into the enzyme, enabling environmentally friendly ambient temperature shipping. Room temperature stability also enables maximum convenience allowing reactions to be set up without ice at room temperature. Applications - High fidelity PCR (sequencing library preparation, cloning, protein expression, site directed mutagenesis, etc.) - Long range PCR (up to 22 kb DNA, depending on the target) - Amplification of both GC- and AT-rich - Workflows using uracil containing DNA templates or requiring dUTP toleration – bisulfite sequencing, damaged DNA (FFPE samples) - Applications requiring high multiplex capabilities Properties - Sample type: DNA - Product format: Master mix - Hot start: Yes, chemical (12–15-minute activation) - Fidelity: >100x compared to Taq polymerase - Detection type: end-point analysis - Overhang: Blunt - Amplicon Size: up to 22 kb DNA - Targets: 1-18+ Mix composition - Solis Hot Start HiFi DNA Polymerase - 10 mM MgCl2 (final concentration 2 mM) - dNTPs - Additives Ordering sizes - 40 rxn | 0.2 ml free sample (Catalog number: 04-44-0000S) - 200 rxn | 1 ml (Catalog number: 04-44-00200) - 1000 rxn | 5 x 1 ml (Catalog number: 04-44-00200-5) - 2000 rxn | 10 x 1 ml (Catalog number: 04-44-00200-10) - 4000 rxn | 20 ml (Catalog number: 04-44-04000) Storage & Shipping - Routine storage: -20 ºC - Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of the reagents - Shipping: At room temperature Characteristics / Technical Specifications - High-fidelity amplification (>100x Taq) - Multiplexing: up to 18+ targets - Long range PCR: up to 22 kb - GC content tolerance: 27–79% - Benchtop stability: up to 24 hours - Room temperature stability: up to 1 month - Hot start: chemical, 12–15 min activation - Inhibitor tolerance: Yes - dUTP tolerance: Yes - Format: 5x Master Mix - Storage: -20°C (routine), RT (1 month)