RNA library preparation reagent kit R2024, R2096

NGS

The Swift Rapid RNA Library Kit offers the fastest, lowest cost NGS transcriptomics workflow for Illumina® sequencing platforms. Leveraging patented Adaptase® technology, this kit enables stranded RNA library construction directly from 1st strand cDNA without the requirement for 2nd strand cDNA synthesis and degradation, or template-switching methods. The kit is compatible with upstream and downstream enrichment and depletion methods, supports a variety of indexing options, and is easily automatable. Save time and cost: At 3.5 hours, this is the fastest RNA library kit on the market. Combined with the lower cost, you can process more samples in less time to increase your data output. Supports many workflows with indexing options, automation, and Normalase: single, combinatorial dual indexing up to 768, unique dual indexing up to 96, and enzymatic library normalization available; automation-friendly. Robust Performance: Maintains ligation efficiency at all supported inputs and no adapter titration required. Robust Mapping and Strandedness The Swift Rapid RNA Library Kit provides competitive metrics for routine transcriptomics, including high mapping rates and maintenance of strandedness. Universal Human Reference (UHR) Total RNA (Agilent 740000) was poly(A) enriched using the NEBNext poly(A) mRNA Magnetic Isolation Module (NEB E7490) before beingn processed through the Swift Rapid RNA Library Kit or process following the supplier’s recommendations. Libraries were sequenced on a MiniSeq with 2 x 75 bp paired-end reads. Fastq files were downsampled to 3.8 million reads before analysis using STAR (mapping rate) or rnaseqc (strandedness).