Solution reagent kit SMART-Seq® v4 Ultra®

for DNA sequencingfor single cell RNA sequencinglead

Single-cell RNA-seq enables researchers to study how gene expression patterns vary within cell populations. Takara Bio has developed robust tools for performing RNA-seq on individual cells isolated in Fluidigm's integrated fluidic circuit (IFC) system. The sensitive SMARTer chemistry of our ultra-low input kits paired with the high-throughput automation capability of the Fluidigm C1 system offer high-resolution insights for transcriptome analysis. We currently offer two generations of single-cell mRNA-seq kits for the Fluidigm C1 Single Cell Auto Prep system. The newest version, the SMART-Seq v4 Ultra Low Input RNA Kit for the Fluidigm C1 System, incorporates features from our industry-leading SMART-Seq v4 kit, including SMART (Switching Mechanism at 5’ End of RNA Template) and locked nucleic acid (LNA) technologies, which are collectively referred to as "SMARTer-seq® chemistry". This kit outperforms published protocols and our previous version, the SMARTer Ultra Low RNA Kit for the Fluidigm C1 System, by providing higher sensitivity, better reproducibility, and an improved representation of GC- and AT-rich genes. The new script for using this kit is supported by the mRNA Seq IFC and the Open App IFC, and can be downloaded through the Fluidigm Script Hub. Overview Improved SMART-technology-based chemistry—LNA technology in the SMART-Seq v4 Oligo and improved chemistry lead to better performance (higher sensitivity, greater reproducibility, and more GC-rich genes detected). Library construction in 2 days—The cDNA synthesis protocol takes approximately two hours, followed by the eight-hour SMART-Seq v4 script on the C1 instrument.