Buffer solution reagent kit SMART-Seq® HT
for DNA sequencingfor single cell RNA sequencingfor cDNA synthesis

buffer solution reagent kit
buffer solution reagent kit
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Characteristics

Type
buffer solution
Applications
for nucleic acids, for DNA sequencing, for cDNA synthesis, for single cell RNA sequencing
Method
enzymatic
Storage temperature

-70 °C, -20 °C
(-94 °F, -4 °F)

Description

The SMART-Seq HT Lysis Components are accessories to the SMART-Seq HT Kit. These components consist of 10X Lysis Buffer as well as the 3′ SMART-Seq CDS Primer II A, and are provided in separate packaging for users who want to use FACS to sort a large number of cells or nuclei without using all of the sorted material as input for cDNA synthesis with the SMART-Seq HT Kit. Overview Easily automate the streamlined workflow—process more samples in less time and maximize the space on your automation deck Unparalleled sensitivity—start with as little as one cell or 10 pg of total RNA (input range: 1–100 cells or 10 pg–1 ng of total RNA) High-quality RNA-seq data—capture full-length transcript coverage, a low percentage of rRNA reads, and a broad representation of GC-rich transcripts Single-tube workflows—complete cDNA synthesis and library preparation, in one tube each, for minimization of sample loss, sample mix-up, or other handling errors Increased sequencing power—include unique dual indexes to allow for the pooling of multiple samples and confident sequencing on the NovaSeq™ system High library yield—sequence on high-throughput sequencers with ease (such as the NovaSeq) or confidently save extra library for future runs More Information Applications cDNA synthesis from single cells or total RNA for transcriptome sequencing cDNA outputs from the SS-HT kit can be used with the SMART-Seq Library Prep kit (not sold separately; sold as part of the SMART-Seq HT PLUS kit) for Illumina sequencing Additional product information Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications.
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