DNA polymerase reagent kit Advantage® HD

antibodyTaq DNA polymerasefor nucleic acids

The Advantage HD Polymerase Mix provides maximum fidelity and extended PCR product length, making it particularly useful for applications that require high accuracy, such as cDNA cloning, site-directed mutagenesis, and mutation genotyping (i.e., SNP analysis). The Advantage HD Polymerase Mix consists of a novel DNA polymerase with a hot-start antibody, and comes with a tube of 5X buffer (with Mg2+). Advantage HD Polymerase Mix provides outstanding accuracy due to the presence of 3' → 5' exonuclease activity that results in an extremely low error rate. It also performs extremely well on GC-rich and other more complex templates. The Advantage HD Polymerase Mix amplifies targets of up to 8.5 kb from human genomic DNA, 10 kb from E. coli genomic DNA, and 22 kb from lambda DNA. The amplification efficiency of Advantage HD Polymerase Mix is greater than that of wild-type Taq polymerase. Advantage HD Polymerase is a hot-start PCR enzyme that is suitable for applications that require high fidelity and sensitivity, such as gene cloning and amplification of cDNAs for library construction. A separate tube of Mg2+ plus buffer is supplied with the polymerase. Sufficient reagents are provided for 200 reactions of 25 μl each. dNTPs need to be purchased separately. Overview Extremely low error rate—exonuclease proofreading yields just 12 errors per 250,000 bp, and performs well on GC-rich templates. Integrated hot-start antibody for enhanced specificity—prevents false initiation during reaction setup and minimizes primer-dimer formation, thus reducing background. High priming efficiency—shortens reaction time and produces results fast.