FT-MS spectrometer Q Exactive™ BioPharma

MSfor protein analysisfor biopharmaceutical applications

Short description
The Thermo Scientific™ Q Exactive™ BioPharma mass spectrometer delivers integrated intact- and subunit-level analysis and high-throughput peptide mapping using a Quadrupole‑Orbitrap analyzer.

Overview
The platform supports complete BioPharma characterization with operational modes tailored to the main protein workflows:

• High Mass Range mode — for intact protein analysis under native and denaturing conditions, optimized for high mass detection.
• Protein mode — for isotopic resolution of subunits and top/middle‑down sequencing.
• Standard mode — for peptide mapping with fast acquisition, high mass accuracy and reliable spectral quality.

Platform variants and software
Q Exactive Plus, Q Exactive HF and Q Exactive HF‑X variants extend native and denatured intact MS and subunit top/middle‑down capabilities. Integration with Thermo Scientific BioPharma Finder software enables combined hardware/software workflows for characterization and data analysis.

Product options (selected)

• Catalog 0726055 — Scan rates: up to 12 Hz; Resolution: 280,000 at m/z 200; Mass range: 50 to 8000 m/z

Documents & resources (selected)

• Poster Note: Full Characterization of Heterogeneous Antibody Samples Under Denaturing and Native Conditions on a Hybrid Quadrupole‑Orbitrap Mass Spectrometer
• Poster Note: Complete Characterization of a Cysteine‑linked Antibody‑Drug Conjugate Performed on a Hybrid Quadrupole‑Orbitrap Mass Spectrometer with High Mass Range
• Application Note: Full Characterization of Heterogeneous Antibody Samples Under Denaturing and Native Conditions on the Q Exactive BioPharma Mass Spectrometer
• Brochure: Q Exactive BioPharma – The Complete BioPharma Characterization Solution

Technical specifications

• Dynamic range: >5000:1
• Mass accuracy: Internal: <1 ppm RMS; External: <3 ppm RMS (under defined conditions)
• Polarity switching: one full cycle <1 s (positive + negative at Resolution 35,000)
• Scan rates: up to 12 Hz
• Sensitivity: Full MS: 500 fg Buspirone on column S/N 100:1; SIM: 30 fg Buspirone on column S/N 100:1
• Mass range: 50 to 8000 m/z
• Resolution: 280,000 at m/z 200
• Type: BioPharma platform