MS spectrometer Q Exactive™ BioPharma

FT-MSfor proteomicsfor protein analysis

Short description
The Thermo Scientific™ Q Exactive™ BioPharma Mass Spectrometer offers superior intact and subunit top/middle-down analysis, as well as peptide mapping, powered by the Orbitrap mass analyzer.

Product overview
Realize complete BioPharma characterization with the Thermo Scientific™ Q Exactive™ BioPharma platform, which offers distinct operational modes optimized for the top three protein characterization workflows:

• High Mass Range mode offers optimized intact protein analysis under both native and denaturing conditions providing the highest quality spectra.
• Protein mode ensures isotopic resolution of subunits and facilitates top/middle-down sequencing.
• Standard mode performs peptide mapping with unparalleled acquisition speed, mass accuracy, and spectral quality.

Platform family and compatibility
The Q Exactive™ BioPharma platform encompasses benchtop hybrid quadrupole-Orbitrap mass spectrometers including Q Exactive™ Plus, Q Exactive™ HF and Q Exactive™ HF-X, enabling denatured and native MS intact analysis, subunit top/middle-down analysis and high-performance peptide mapping workflows. When combined with Thermo Scientific™ BioPharma Finder™ software it provides an integrated hardware and software solution for BioPharma characterization.

Operational modes and use cases

• High Mass Range (HMR) mode: designed for intact therapeutic protein workflows, optimized for conventional denaturing intact monoclonal antibody analysis and native MS analysis ideal for antibody drug conjugates (ADCs) and other heterogeneous antibody samples.
• Protein mode: provides high-quality isotopically resolved data for subunit deconvolution and top/middle-down sequencing.
• Standard mode: state-of-the-art peptide mapping using refined data dependent acquisition (DDA) for rapid, accurate peptide-level characterization.

Additional capabilities
The platform supports high acquisition speed and high-resolution performance suitable for intact protein, subunit and peptide workflows, and is designed to be integrated into BioPharma characterization workflows with dedicated software support.

Technical specifications

• Catalog Number: 0726055
• Type: BioPharma Platform
• Scan Rates: Up to 12 Hz
• Resolution: 280,000 at m/z 200
• Mass Range: 50 to 8000 m/z
• Dynamic Range: >5000:1
• Mass Accuracy: Internal: <1 ppm RMS; External: <3 ppm RMS under defined conditions
• Polarity Switching: One full cycle in <1 sec (one full positive mode scan and one full negative mode scan at resolution setting of 35,000)
• Sensitivity: Full MS: 500 fg Buspirone on column S/N 100:1; SIM: 30 fg Buspirone on column S/N 100:1
• Unit Size: Each