Human bocavirus reagent kit 7041023

solutionenzymebuffer solution

Vitassay qPCR Kits contains in each well enzymes, buffer, primer and probes as well as an internal control in a lyophilized format. The kits include a positive and a negative control. Identification of pathogen DNA or RNA occurs by the use of target specific primers ans fluorecent labeled hydrolysis probes which hybridize conserved regions in the genomes of the differentpathogens. Real-time PCR kit for the qualitative detection of Bocavirus in human samples Intended use Vitassay qPCR Bocavirus allows the detection of Bocavirus by real-time PCR in clinical samples. The product is intended for use in the diagnosis of Bocavirus infections alongside clinical data of the patient and other laboratory tests outcomes. Transport and storage date stated in the label. • The resuspended positive control should be stored at -20ºC. In order to avoid repeated freeze/thaw cycles, it is recommended to distribute the content in different aliquots. • Keep all reagents in the darkness. Additional equipment and material required • DNA extraction kit • Real-time PCR instrument (thermocycler) (Attached I) • Centrifuge for 1.5 ml tubes • Vortexer • Micropipettes (1-20 µl, 20-200 µl) • Filter tips • Powder-free disposal gloves Summary Acute viral respiratory tract infections are the leading cause of hospitalization for infants and young children in developed countries, whilst a major cause of death in developing countries. The human bocavirus (HBoV) is a newly, globally distributed, discovered parvovirus which belongs to the Parvoviridae family and the Bocavirus genus. Characterized for being a single-stranded DNA virus, it has four different genotypes.