Membrane ultrafilter Vivacon® 2

for liquidsfor protein solutionslaboratory

Overview
Vivacon® 2 concentrators process up to 2 ml starting volume and enable concentration up to 30×. Designed for rapid DNA concentration and PCR primer removal; also suitable for peptide fractionation by filter-aided sample preparation (FASP). Devices use low-binding Hydrosart® regenerated cellulose membranes mounted horizontally for optimized DNA handling. Selected MWCO variants are available ethylene oxide (ETO) sterilized and free of detectable DNA. Compatible with fixed-angle rotors that accept 15 ml conical tubes (17 mm × 95 mm).

Applications

• DNA rebuffering after organic extraction prior to sequencing.
• Removal of dyes and reagents after sequencing reactions.
• PCR primer removal and cleanup.
• Plasmid concentration and desalting.

Performance — Vivacon® 2 for DNA

• 2,000 MWCO — ~10 bp — 120 min — 92% recovery — 7,500 xg
• 10,000 MWCO — ~30 bp — 60 min — 94% recovery — 5,000 xg
• 30,000 MWCO — ~50 bp — 60 min — 95% recovery — 2,500 xg
• 50,000 MWCO — ~300 bp — 45 min — 96% recovery — 2,500 xg
• 100,000 MWCO — ~600 bp — 30 min — 93% recovery — 2,500 xg
• 125,000 MWCO (Cellulose Triacetate) — ~650 bp — 30 min — 88–89% recovery — 2,000 xg

Performance — Vivacon® 2 for proteins

• 2,000 MWCO — 0.25 mg/ml cytochrome c — 120 min — 95% recovery — 7,500 xg
• 10,000 MWCO — 0.25 mg/ml cytochrome c — 90 min — 96% recovery — 5,000 xg
• 30,000 MWCO — 1.0 mg/ml BSA — 40 min — 96% recovery — 5,000 xg
• 50,000 MWCO — 1.0 mg/ml BSA — 30 min — 94% recovery — 5,000 xg
• 100,000 MWCO — 1.0 mg/ml bovine IgG — 30 min — 92% recovery — 5,000 xg
• 125,000 MWCO — 1.0 mg/ml bovine IgG — 27 min — 81% recovery — 5,000 xg

Conversion Table

• Hydrosart® — 2 kDa — > 10 bp
• Hydrosart® — 10 kDa — > 30 bp
• Hydrosart® — 30 kDa — > 50 bp
• Hydrosart® — 50 kDa — > 300 bp
• Hydrosart® — 100 kDa — > 600 bp
• Cellulose Triacetate — 125 kDa — > 650 bp

Technical information
Design features a horizontal membrane and reverse-spin recovery. Intended for fixed-angle rotors fitting 15 ml conical bottom tubes (17 mm diameter, 95 mm depth). Maximum recommended speed: 2,500 xg (may be increased in specific cases; DNA recovery can vary with g-force). Hold-up volume (membrane and support) < 10 µl. Dead stop volume for fixed-angle rotor (25°) ~55 µl; no dead stop with swing-bucket rotors. Refer to manual for rotor-specific guidance and extended operating conditions.

Technical specifications

• Concentrator capacity (fixed-angle rotor): 2 ml
• Total length (concentration): 125 mm
• Total length (backspin): 115 mm
• Width: 16 mm
• Active membrane area: 0.95 cm²
• Hold-up volume (membrane and support): < 10 µl
• Dead stop volume (fixed-angle, 25° rotor): 55 µl
• Dead stop volume (swing-bucket rotor): No dead stop
• Body material: Polycarbonate
• Filtrate vessel: Polypropylene
• Back spin vial: Polypropylene
• Concentrator cap: Polypropylene
• O-ring: Silicone
• Membrane: Hydrosart® (125,000 MWCO uses Cellulose Triacetate)
• Rotor type required: Fixed-angle (fits 15 ml / 17 mm conical bottom tubes, 95 mm depth)
• Maximum speed: 2,500 xg (see manual for details)