Membrane ultrafilter Vivacon® 500

for liquidsfor protein solutionslaboratory

Product overview
Vivacon® 500 centrifugal concentrator with a horizontal, low‑binding Hydrosart® regenerated cellulose membrane optimized for DNA and general biomolecule concentration. Designed for starting volumes up to 500 µl and concentration factors up to 50×. Selected MWCOs are available ETO‑treated for sterile, PCR‑grade devices. Compatible with microcentrifuges using fixed‑angle rotors that accept 1.5 / 2.2 ml conical bottom tubes (95 mm depth).

Key features

• Start volume: up to 500 µl
• Concentration: up to 50×
• Horizontal, low‑binding Hydrosart® regenerated cellulose membrane (most MWCOs)
• 125,000 MWCO: Cellulose Triacetate membrane
• ETO‑treated options for sterile, DNA‑free devices
• Compatible with fixed‑angle rotors fitting 1.5 / 2.2 ml conical bottom tubes
• Reverse‑spin (backspin) recovery for maximum sample retrieval

Applications

• DNA concentration and rebuffering prior to sequencing
• Primer and dye removal after sequencing reactions
• Plasmid concentration
• Protein concentration and rebuffering
• Small‑volume sample concentration for downstream assays

Performance — Vivacon® 500 for DNA (start volume 0.5 ml, sample 50 ng/ml)

• 2,000 MWCO — Time to 30×: ~60 min at 20°C — Recovery: 93% — g‑force: 7,500 xg
• 10,000 MWCO — Time to 30×: ~25 min at 20°C — Recovery: 94% — g‑force: 7,500 xg
• 30,000 MWCO — Time to 30×: ~18 min at 20°C — Recovery: 88% — g‑force: 5,000 xg
• 50,000 MWCO — Time to 30×: ~18 min at 20°C — Recovery: 91% — g‑force: 3,000 xg
• 100,000 MWCO — Time to 30×: ~10 min at 20°C — Recovery: 87% — g‑force: 3,000 xg
• 125,000 MWCO (CTA) — Time to 30×: ~12 min at 20°C — Recovery: 85% — g‑force: 2,000 xg
• 125,000 MWCO (alt) — Time to 30×: ~9 min at 20°C — Recovery: 94% — g‑force: 3,000 xg

Performance — Vivacon® 500 for proteins (start volume 0.5 ml)

• 2,000 MWCO — 0.25 mg/ml cytochrome c — Time to 30×: ~30 min at 20°C — Recovery: 95% — g‑force: 14,000 xg
• 10,000 MWCO — 0.25 mg/ml cytochrome c — Time to 30×: ~15 min at 20°C — Recovery: 92% — g‑force: 14,000 xg
• 30,000 MWCO — 1.0 mg/ml BSA — Time to 30×: ~10 min at 20°C — Recovery: 95% — g‑force: 14,000 xg
• 50,000 MWCO — 1.0 mg/ml BSA — Time to 30×: ~10 min at 20°C — Recovery: 92% — g‑force: 14,000 xg
• 100,000 MWCO — 1.0 mg/ml bovine IgG — Time to 30×: ~11 min at 20°C — Recovery: 90% — g‑force: 8,000 xg
• 100,000 MWCO — 0.02 mg/ml porcine hematin — Time: ~10 min (note) — Recovery: 88% — g‑force: 2,500 xg
• 125,000 MWCO — 1.0 mg/ml bovine IgG — Time to 30×: ~10 min at 20°C — Recovery: 81% — g‑force: 8,000 xg
• 125,000 MWCO — 0.02 mg/ml porcine hematin — Time: ~10 min (note) — Recovery: 11% — g‑force: 2,500 xg

Conversion (membrane vs nucleotide cut‑off)

• Hydrosart® 2 kDa — double‑stranded nucleotide cut‑off: >10 bp
• Hydrosart® 10 kDa — cut‑off: >30 bp
• Hydrosart® 30 kDa — cut‑off: >50 bp
• Hydrosart® 50 kDa — cut‑off: >300 bp
• Hydrosart® 100 kDa — cut‑off: >600 bp
• Cellulose Triacetate 125 kDa — cut‑off: >650 bp

Notes
Reverse‑spin (backspin) recovery is available. Hold‑up volume (membrane and support) <5 µl; dead‑stop volume 5 µl (40° rotor). Active membrane area: 0.32 cm². Maximum recommended speed: 5,000 xg (in some cases higher speeds may be used; DNA recovery can improve at lower g forces).

Technical specifications

• Concentrator capacity (Fixed Angle Rotor): 0.5 ml
• Total length (concentration): 45 mm
• Total length (backspin): 47.5 mm
• Width: 12.4 mm
• Active membrane area: 0.32 cm²
• Hold‑up volume (membrane and support): <5 µl
• Dead‑stop volume: 5 µl (40° rotor)
• Body material: Polycarbonate
• Filtrate vessel: Polypropylene
• Back spin vial: Polypropylene
• O‑ring: Silicone
• Membrane (except 125,000 MWCO): Hydrosart®
• Membrane (125,000 MWCO): Cellulose Triacetate
• Required rotor type: Fixed Angle (minimum rotor angle 40°)
• Rotor cavity: fits 1.5 / 2.2 ml (11 mm) conical bottom tubes (95 mm depth)
• Maximum speed: 5,000 xg (may be increased in some cases; monitor DNA recovery)