Membrane ultrafilter Vivacon® 500 ETO

for liquidsfor protein solutionslaboratory

Product overview
Vivacon® 500 ETO centrifugal concentrators enable rapid concentration of nucleic acids and removal of PCR primers from samples with a 0.5 ml starting volume. Concentration factors up to 50x using low‑binding Hydrosart® regenerated cellulose horizontal membranes. Compatible with microcentrifuges having fixed‑angle rotors that accept 1.5 / 2.2 ml (11 mm) conical bottom tubes (95 mm depth). Reverse spin function simplifies sample recovery. Dual‑cycle ethylene oxide (ETO) sterilization to SAL 10⁻⁶; tested free of detectable human genomic DNA by qPCR (55 cycles; detection limit 2 pg).

Applications

• DNA rebuffering of organic extraction samples prior to sequencing.
• Dye removal after sequencing reactions.
• Primer removal from PCR products.
• Plasmid concentration and clean‑up.

Performance — Vivacon® 500 for DNA

• 2,000 MWCO — Sample size (bp): 10 — Time to concentrate up to 30x: 60 min at 20°C — Concentrate recovery: 93% — g‑force: 7,500 xg
• 10,000 MWCO — Sample size (bp): 30 — Time: 25 min at 20°C — Recovery: 94% — g‑force: 7,500 xg
• 30,000 MWCO — Sample size (bp): 50 — Time: 18 min at 20°C — Recovery: 88% — g‑force: 5,000 xg
• 50,000 MWCO — Sample size (bp): 300 — Time: 18 min at 20°C — Recovery: 91% — g‑force: 3,000 xg
• 100,000 MWCO — Sample size (bp): 600 — Time: 10 min at 20°C — Recovery: 87% — g‑force: 3,000 xg
• 125,000 MWCO — Sample size (bp): 650 — Time: 12 min at 20°C — Recovery: 85% — g‑force: 2,000 xg
• 125,000 MWCO — Sample size (bp): 900 — Time: 9 min at 20°C — Recovery: 94% — g‑force: 3,000 xg

Performance — Vivacon® 500 for proteins

• 2,000 MWCO — 0.25 mg/ml cytochrome c — Time to concentrate up to 30x: 30 min at 20°C — Recovery: 95% — g‑force: 14,000 xg
• 10,000 MWCO — 0.25 mg/ml cytochrome c — Time: 15 min at 20°C — Recovery: 92% — g‑force: 14,000 xg
• 30,000 MWCO — 1.0 mg/ml BSA — Time: 10 min at 20°C — Recovery: 95% — g‑force: 14,000 xg
• 50,000 MWCO — 1.0 mg/ml BSA — Time: 10 min at 20°C — Recovery: 92% — g‑force: 14,000 xg
• 100,000 MWCO — 1.0 mg/ml bovine IgG — Time: 11 min at 20°C — Recovery: 90% — g‑force: 8,000 xg
• 100,000 MWCO — 0.02 mg/ml porcine hematin — Time: 10 min at 20°C — Recovery: 88% — g‑force: 2,500 xg
• 125,000 MWCO — 1.0 mg/ml bovine IgG — Time: 10 min at 20°C — Recovery: 81% — g‑force: 8,000 xg
• 125,000 MWCO — 0.02 mg/ml porcine hematin — Time: 10 min at 20°C — Recovery: 11% — g‑force: 2,500 xg

Conversion table

• Hydrosart® — 2 kDa — Double‑stranded nucleotide cut‑off (bp): >10
• Hydrosart® — 10 kDa — Cut‑off: >30 bp
• Hydrosart® — 30 kDa — Cut‑off: >50 bp
• Hydrosart® — 50 kDa — Cut‑off: >300 bp
• Hydrosart® — 100 kDa — Cut‑off: >600 bp
• Cellulose Triacetate — 125 kDa — Cut‑off: >650 bp

Ordering / available variants

• VN01H22ETO — PCR Grade, 30,000 MWCO, Hydrosart membrane, 100 per pack — $636.00
• VN01H23ETO — PCR Grade, 30,000 MWCO, Hydrosart membrane, 500 per pack — Discontinued (order VN01H22ETO instead)
• VN01H42ETO — PCR Grade, 100,000 MWCO, Hydrosart membrane, 100 per pack — $636.00
• VN01H43ETO — PCR Grade, 100,000 MWCO, Hydrosart membrane, 500 per pack — Discontinued (order VN01H42ETO instead)

Technical specifications

• Concentrator capacity (fixed angle rotor): 0.5 ml
• Total length (concentration): 45 mm
• Total length (backspin): 47.5 mm
• Width: 12.4 mm
• Active membrane area: 0.32 cm²
• Hold‑up volume (membrane and support): <5 µl
• Dead stop volume: 5 µl (40° rotor)
• Body material: Polycarbonate
• Filtrate vessel: Polypropylene
• Back spin vial: Polypropylene
• O‑ring: Silicone
• Membrane (except 125,000 MWCO): Hydrosart® (regenerated cellulose)
• Membrane (125,000 MWCO): Cellulose Triacetate
• Required rotor type: Fixed angle (minimum rotor angle 40°)
• Rotor cavity: Fits 1.5 / 2.2 ml (11 mm) conical bottom tubes (95 mm depth)
• Maximum recommended speed: 5,000 xg (may be increased in some cases; see manual)
• Sterilization / contamination control: Dual‑cycle ETO sterilization to SAL 10⁻⁶; tested for absence of detectable human genomic DNA by qPCR (55 cycles; detection limit 2 pg)