Membrane ultrafilter Vivacon® 2 ETO

for liquidsfor protein solutionslaboratory

Product overview
Vivacon® 2 ETO centrifugal concentrators enable rapid DNA concentration and PCR primer removal for samples up to 2 ml. They use low-binding Hydrosart® regenerated cellulose membranes positioned horizontally for optimal recovery. Compatible with fixed-angle rotors that accept 15 ml centrifuge tubes. Reverse spinning into the upper recovery cap simplifies sample retrieval. Devices are sterilized by a dual-cycle ethylene oxide (ETO) gas process to a SAL of 10⁻⁶ and tested by qPCR (55 cycles) with a detection limit of 2 pg, ensuring no detectable human genomic DNA contamination.

Key features

• Maximum starting volume: 2 ml.
• Concentration factor: up to 30×.
• Low-binding Hydrosart® membranes (various MWCO up to 100 kDa) and cellulose triacetate option (125 kDa).
• Designed for use in fixed-angle rotors fitting 15 ml conical tubes; backspin recovery cap for simplified retrieval.
• ETO sterilized (dual-cycle), PCR-grade.

Applications

• DNA rebuffering of organic extraction samples prior to sequencing.
• Dye removal after sequencing reactions.
• Primer removal and plasmid concentration.
• Concentration of nucleic acids and proteins for downstream analysis.

Performance - Vivacon® 2 for DNA

• 2,000 MWCO — Time to concentrate up to 30×: 120 min at 20°C | Concentrate recovery: 92% | g-force: 7,500 xg
• 10,000 MWCO — Time: 60 min at 20°C | Recovery: 94% | g-force: 5,000 xg
• 30,000 MWCO — Time: 60 min at 20°C | Recovery: 95% | g-force: 2,500 xg
• 50,000 MWCO — Time: 45 min at 20°C | Recovery: 96% | g-force: 2,500 xg
• 100,000 MWCO — Time: 30 min at 20°C | Recovery: 93% | g-force: 2,500 xg
• 125,000 MWCO (Cellulose Triacetate) — Time: 30 min at 20°C | Recovery: 88–89% | g-force: 2,000 xg

Performance - Vivacon® 2 for proteins

• 2,000 MWCO — 0.25 mg/ml cytochrome c | Time to concentrate up to 30×: 120 min at 20°C | Recovery: 95% | g-force: 7,500 xg
• 10,000 MWCO — 0.25 mg/ml cytochrome c | Time: 90 min | Recovery: 96% | g-force: 5,000 xg
• 30,000 MWCO — 1.0 mg/ml BSA | Time: 40 min | Recovery: 96% | g-force: 5,000 xg
• 50,000 MWCO — 1.0 mg/ml BSA | Time: 30 min | Recovery: 94% | g-force: 5,000 xg
• 100,000 MWCO — 1.0 mg/ml bovine IgG | Time: 30 min | Recovery: 92% | g-force: 5,000 xg
• 125,000 MWCO — 1.0 mg/ml bovine IgG | Time: 27 min | Recovery: 81% | g-force: 5,000 xg

Conversion table

• Hydrosart® — 2 kDa MWCO — Double-stranded nucleotide cut-off: > 10 bp
• Hydrosart® — 10 kDa MWCO — Cut-off: > 30 bp
• Hydrosart® — 30 kDa MWCO — Cut-off: > 50 bp
• Hydrosart® — 50 kDa MWCO — Cut-off: > 300 bp
• Hydrosart® — 100 kDa MWCO — Cut-off: > 600 bp
• Cellulose Triacetate — 125 kDa MWCO — Cut-off: > 650 bp

Ordering information
PCR Grade, 100,000 MWCO, Hydrosart® membrane, 100 per pack (Prod. #: VN02H42ETO) Price: $848.00

Specifications

• Concentrator capacity (fixed-angle rotor): 2 ml.
• Total length: 125 mm (concentration) / 115 mm (backspin); Width: 16 mm.
• Active membrane area: 0.95 cm².
• Hold-up volume (membrane and support): <10 µl.
• Dead stop volume (fixed-angle rotor 25°): 55 µl; swing-bucket rotor: no dead stop.
• Materials: Body – polycarbonate; Filtrate vessel – polypropylene; Backspin vial – polypropylene; Concentrator cap – polypropylene; O-ring – silicone.
• Membrane types: Hydrosart® (various MWCO up to 100 kDa); Cellulose Triacetate (125 kDa).
• Equipment required: Centrifuge with fixed-angle rotor fitting 15 ml (17 mm) conical bottom tubes (95 mm depth).
• Maximum recommended speed: 2,500 xg (may be increased in some cases; DNA recovery can vary with g-force).