OverviewThe Pannoramic 150 is a dual-mode digital slide scanner for high-throughput brightfield and fluorescence imaging in research laboratories. It combines a continuously moving stage, Plan-Apochromat optics and a back-illuminated sCMOS fluorescence camera to deliver high-resolution images with automation suited to long unattended runs.
Key Features- 150-slide capacity (6 magazines × 25) with continuous loading to minimize workflow interruptions.
- Dual brightfield and fluorescence scanning with automated barcode reading and tissue detection.
- High-speed brightfield: dedicated 5 MP color CMOS camera (72 fps) and Plan-Apochromat 40x/0.95 NA objective, up to 103x effective magnification at 0.10 µm/pixel.
- Accelerated fluorescence scanning using a 10x fluorescence Plan-Apochromat objective and a back-illuminated 16‑bit 4.2 MP sCMOS camera for improved sensitivity.
- 9-position motorized fluorescence filter changer with multiband support and high-end light engine for multiplexing.
- Motorized camera and (optional) objective changers for automated transitions between modes and magnifications.
- Server-side barcode reading and imaging software integration for centralized data management.
Imaging and Scanning Highlights- Brightfield: up to 103x (40x objective + 1.6x adapter); resolution down to 0.10 µm/pixel with 40x objective.
- Brightfield camera: 5 MP color CMOS, 72 fps for high-fidelity color capture.
- Brightfield illumination: LED transmitted light with LED FLASH technology (~60,000 h life).
- Fluorescence: 10x Plan-Apochromat reduces scan time by ~50%; fluorescence resolution to 0.10 µm/pixel with 40x+1.6x adapter.
- Fluorescence illumination: Lumencor SOLA, Spectra III or CoolLED pE-800 covering ~380–740 nm.
- Fast multicolor preview/flash scanning for sample detection and automated tissue/coverslip identification.
Automation, Throughput & Workflow- Continuous automatic loading for long unattended runs; 150-slide capacity (6 × 25).
- Optimized throughput for high-volume research: brightfield example ~1 min 30 s for 15 × 15 mm at 0.19 µm/pixel.
- Fluorescence example ~8 min 30 s for 15 × 15 mm with 3 channels (20 ms exposure).
- Automated focus routines, flat-field correction, deconvolution and USB preview camera for reliable tissue detection.
- Optional Z-stack scanning (1–30 layers, 0.2–2 µm step) for extended focus and 3D analysis.
Compatibility & Output- Slide compatibility: 75.0–76.2 mm × 25.0–26.0 mm × 0.9–1.2 mm; coverslips No.1 or No.1.5 (0.13–0.19 mm).
- Image formats: native MRXS with export to JPG, BMP, PNG, TIFF, SVS and DICOM.
- Barcode support: 1D and 2D (Code39, Code128, QR, Data Matrix) for sample tracking.
Use Cases- Designed for research and high-throughput labs: biomedical and cancer research, genetics, plant sciences and other research workflows requiring combined brightfield and fluorescence imaging.
Available Objectives & Options- Objectives: Plan-Apochromat 10x/0.3 (fluorescence option), 20x/0.8 (default), 40x Corr/0.95.
- Optional 1.6x adapter to increase effective magnification for both modes; motorized objective changer available when two objectives are selected.
- Optional features: multilayer (Z-stack) scanning, extended focus scanning, additional software modules.
Physical & Operational- Approx. dimensions: 673 × 689 × 546 mm (scanner unit).
- Approx. weight: 49 kg (scanner unit).
Technical Specifications- Model: Pannoramic 150
- Manufacturer / Brand: 3DHISTECH
- Slide capacity: 150 slides (6 × 25)
- Scanning modes: Brightfield and Fluorescence (dual-mode)
- Brightfield camera: 5 MP color CMOS, 72 fps
- Fluorescence camera: Back-illuminated 16-bit 4.2 MP sCMOS
- Fluorescence filter changer: 9-position motorized, multiband support
- Objectives: 10x/0.3 (fluorescence opc.), 20x/0.8 (default), 40x/0.95 (Plan-Apochromat)
- Maximum brightfield magnification: up to 103x (with 40x objective)
- Fluorescence magnification: up to ~98x (with 1.6x adapter)
- Resolution: down to 0.10 µm/pixel (with 40x objective)
- Brightfield example speed: ~1 min 30 s for 15 × 15 mm at 0.19 µm/pixel
- Fluorescence example speed: ~8 min 30 s for 15 × 15 mm with 3 channels (20 ms exposure)
- Illumination (brightfield): LED transmitted light with LED FLASH technology (~60,000 h lifetime)
- Illumination (fluorescence): Lumencor SOLA / Spectra III or CoolLED pE-800 (380–740 nm)
- Automation: Continuous loading, automated barcode reading, tissue detection, intelligent autofocus, flat-field correction
- Z-stack / focus layering: optional, 1–30 layers, 0.2–2 µm step
- Image formats / export: MRXS native; exports to JPG, BMP, PNG, TIFF, SVS, DICOM
- Slide compatibility: 75.0–76.2 mm × 25.0–26.0 mm × 0.9–1.2 mm; No.1 or No.1.5 coverslips (0.13–0.19 mm)
- Barcode types supported: Code39, Code128, QR, Data Matrix
- Dimensions (approx.): 673 × 689 × 546 mm
- Weight (approx.): 49 kg