Enzyme reagent 119630HC

DNA polymeraseTaq DNA polymerasefor real-time PCR

Short description
AptaHot Taq DNA Polymerase HC is an aptamer-based HotStart Taq DNA polymerase (30 U/µl) designed for room-temperature reaction setup and reliable activation during thermal cycling. The high-concentration HC variant is suitable for lyophilization and diagnostic kit manufacturing. Not available via the internet shop.

Details
AptaHot Taq employs DNA aptamers that reversibly dissociate from the enzyme above 50 °C, providing HotStart control without antibodies. The recombinant enzyme amplifies fragments up to 5 kb with high specificity, reducing unspecific products and primer-dimers. It performs well on complex genomic and cDNA templates. The enzyme is supplied with three different 10x buffers (with and without MgCl2) and additional MgCl2 for universal application.

Applications

• Standard PCR
• Real-time PCR (qPCR)
• Multiplex PCR
• Diagnostic kit development and production

Additional information (table)
super-hot-taq-size : 1000U

Technical specifications

• Denomination: AptaHot Taq DNA Polymerase HC
• HotStart mechanism: DNA-aptamers (reversibly dissociate above 50 °C)
• Concentration: 30 U/µl (high-concentrated variant)
• Amplifiable fragment length: up to 5 kb
• Specificity: high, minimizes unspecific products and primer-dimers
• Templates: suitable for complex genomic and cDNA templates
• Buffers supplied: three different 10x buffers (with and without MgCl2) plus additional MgCl2
• Intended uses: standard PCR, real-time PCR, multiplex PCR, diagnostic applications
• Packaging/variant on page: 1000U
• Availability note: not available via internet shop