Protease reagent 405202

proteinase Kenzymefor nucleic acids

Short description
Proteinase K is a broad-spectrum serine protease designed for efficient nucleic acid purification. RNase- and DNase-free; supplied as lyophilized powder or ready-to-use solution.

Description
Proteinase K is a non-specific serine protease with a high specific activity of 30 U/mg. The enzyme retains activity and stability in the presence of SDS, EDTA and chaotropic salts. Supplied free of RNase and DNase activities. The lyophilized powder is highly soluble (> 50 mg/mL); standard stock concentration is 20 mg/mL. Lyophilized material is stable at 15–25 °C for up to 4 days; for long-term storage, store at -20 °C in storage buffer. BIORON also offers Proteinase K Solution at 20 mg/mL.

Application

• Isolation of nucleic acids from bacteria
• Purification of nucleic acids from cells or tissue for PCR and downstream molecular biology

Available sizes
200 mg, 1 g

Technical specifications

• Enzyme class: non-specific serine protease
• Specific activity: 30 U/mg
• Free of RNase and DNase activities
• High stability and activity in presence of SDS, EDTA and chaotropic salts
• Recommended working concentration: 50–100 µg/mL for typical nucleic acid extraction
• Lyophilized powder stability: stable at 15–25 °C for up to 4 days
• Long-term storage: -20 °C in storage buffer recommended
• Solubility: > 50 mg/mL; standard stock concentration 20 mg/mL
• Also available as Proteinase K Solution, 20 mg/mL (ready-to-use)
• Packaging options: 200 mg, 1 g