DescriptionProteinase K is a non-specific serine protease with very high specific activity (30 U/mg). The enzyme retains activity and stability in the presence of SDS, EDTA and chaotropic salts. It is free of RNase and DNase activities. This ready-to-use solution has a concentration of 20 mg/mL; Proteinase K is also available as lyophilized powder. Store at −20 °C.
ApplicationIsolation of nucleic acids from bacteria
Isolation and purification of nucleic acids from cells or tissue for PCR and downstream molecular biology workflows
DatasheetsDatasheet available: IFU_405102_Prot-K-Sol_Rev01_0819_rj.pdf
Characteristics / technical specifications- Concentration: 20 mg/mL
- Specific activity: 30 U/mg
- Enzyme type: non-specific serine protease
- RNase- and DNase-free
- Stable and active in presence of SDS, EDTA and chaotropic salts
- Recommended working concentration: 50–100 µg/mL
- Storage: −20 °C
- Available pack sizes: 1 mL; 5 × 1 mL
- REF / SKU: 405102
- Format: solution (also available as lyophilized powder)