EliGene® Chlamydia trachomatis LC kit is intended for DNA diagnostics of pathogenic bacteria Chlamydia trachomatis from clinical specimens. The kit is based on detection of cryptic plasmid together with genomic DNA of Chlamydia trachomatis bacteria. The kit gives positive result for bacteria Chlamydia trachomatis when also “Sweden variant“ carrying 377 bp deletion in cryptic plasmid and variant lacking cryptic plasmid are detected.
Principle of the method
This diagnostic kit is based on RealTime PCR method using Molecular Beacons hybridization probes. In this kit primers and Molecular Beacons labeled probes (PULSAR and FAM) for the DNA detection of Chlamydia trachomatis and internal control are used.
Chlamydia is gram-negative bacteria that parasitize inside the susceptible cells due to their disability to synthesise ATP. Genus Chlamydia covers four known species: C. trachomatis, C. psittaci, C. pecorum and C. pneumoniae when C. psittaci and C. pecorum primary belongs to animal parasites. Chlamydia trachomatis is the most frequently reported sexually transmitted pathogen when up to 10 % of population is infected. Chlamydia trachomatis is responsible for several clinically different diseases as cervicitis, pelvic inflammatory disease (PID), infant pneumonia and conjunctivitis (pink eye) in newborns, urethritis, prostatitis or epididymitis. In men Chlamydia trachomatis often cause non-gonococcal urethritis. In women, untreated infection can cause serious problems mainly due to the fact that app. 75 % of these infections are asymptomatic.