Trypsin-EDTA reagent 091689649

enzymesolutionHBSS

0.25% Trypsin and 1mM EDTA in modified HBSS w/o Ca++ and Mg++, 100 mL Molecular Weight: Approximately 23;000 (trypsin) Packaging size: 100 mL Product Description Trypsin-EDTA solutions are used to release adherent cells from culture surfaces. This 0.25% Trypsin-EDTA solution is a porcine pancreas-derived enzyme that is commonly used for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture. Ethylenediaminetetracetic acid (EDTA), a chelating agent, is added to trypsin solutions to enhance enzymatic activity by neutralizing calcium and magnesium ions that enhance cell-to-cell adhesion and obscure the peptide bonds on which trypsin acts. Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. Specifications Molecular Weight - Approximately 23;000 (trypsin) Pack Size - 100 mL pH - 7.0 - 7.3 Source - Porcine (for trypsin) Specific Activity - 1 TAME unit = 19.2 USP or NF units = 57.5 BAEE Units Specificity - Specificity: The protease activity of trypsin is highly specific toward positively charged side chains with lysine and arginine. Forms complexes with α2-macroglobulin. Can be used in the isolation of intact; detergent-free phycobilisomes and in the hydrolysis/condensation of carboxylic ester bonds. Sterility - Sterile Unit Definition - One BAEE unit will produce a ΔA253 of 0.001 per minute at pH 7.6 at 25 °C using BAEE as substrate. Reaction volume = 3.2 mL (1 cm light path).