Trypan Blue is a blue acid dye with a strong affinity for cellulose. Trypan blue solution may be used in trypan blue based cytotoxitiy and proliferation assays. Trypan blue is also recommended for use in dye exclusion procedures for viable cell counting. Non-viable cells will up-take trypan blue at a faster rate than viable cells.
Synonyms
Direct Blue 14; Niagara Blue 3B; Chlorazol blue 3B; Benzo blue 3B; Dianil blue H3G; Congo blue 3B; Naphthamine blue 3BX; Benzamine blue 3B; Azidine blue 3B; Diamine Blue 3B;
Molecular Formula: C34H24N6Na4O14S4
Molecular Weight: 960.82
Packaging size: 100 mL
Product Description
Trypan Blue is a blue acid dye with a strong affinity for cellulose containing substrates such as cotton; less affinity for proteinaceous materials. Trypan blue solution may be used in trypan blue based cytotoxitiy and proliferation assays. Trypan Blue is used as a vital dye which is especially important because it is taken up by the reticuloendothelial system. Clark describes assays for the study of teratogenic action of trypan blue on embryonic tissues using Davis and Sauter's fluorescence method and for the staining of collagen, including very fine fibrils, muscle and cornified epithelium using the Van Gieson method. Trypan blue is also recommended for use in dye exclusion procedures for viable cell counting. Non-viable cells will up-take trypan blue at a faster rate than viable cells.
Application Notes
Trypan Blue is used as a vital dye which is especially important because it is taken up by the reticuloendothelial system. Clark describes assays for the study of teratogenic action of trypan blue on embryonic tissues using Davis