Sodium reagent LymphoSep™

solutionplasmafor research

Lymphocyte Separation Medium is a sterile, iso-osmotic, low viscosity solution for density centrifugation yielding a clear separation of lymphocytes from heparinized or defibrinated human blood. Product Description Lymphosep - Lymphocyte Separation Medium is a sterile, iso-osmotic, low viscosity solution for density centrifugation yielding a clear separation of lymphocytes from heparinized or defibrinated human blood. Lymphocytes are isolated by layering diluted, heparinized blood over a solution of sodium diatrizoate that contains a polysaccharide. This polysaccharide aggregates the erythrocytes so that they can be sedimented by low centrifugation. The less dense lymphocytes remain as a clearly defined band at the plasma Lymphosep interface. Maximum yield of mononuclear cells Easy one-step centrifugation >96% cell viability of lymphocytes Low endotoxins Autoclave sterilization Application Notes LSM is formulated for isolation of mononuclear cells from defibrinated or heparinized whole human blood. One-step centrifugation permits separation of mononuclear lymphocytes from erythrocytes, polynuclear lymphocytes and most platelets. Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. Specifications Formulation Details - Liquid - Lymphosep is supplied as a ready made sterile aqueous solution of sodium diatrizoate (9.6% w/v) and Ficoll® (5.6% w/v). pH - 6.0 - 9.0 Sterility - Sterile