DMEM reagent 091642149

glutaminefor researchglucose

Also known as DME, Dulbecco's Modified Eagle's Medium is the most widely used modification of Eagle's Basal Medium (BME). DMEM contains four times greater concentration of amino acids, vitamins and supplementary components. The original formulation calls for 1000 mg/L of glucose, which was first employed to support the polyoma virus in primary and secondary embryonic mouse cultures. For optimal culturing of other cell types, a modification of 4500 mg/L glucose is available from MP. Application Notes 1× Dulbecco's Modification of Eagle's Medium, with 4.5 g/L Dextrose, without Glutamine and L-Leucine and Sodium Pyruvate is ideally suited for supporting and maintaining a vast range of mammalian cell types. For use during SILAC™ protein labeling with stable isotopic labeled lysine and/or arginine. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. Key Applications Mammalian Cell Culture | Ideally suited for supporting and maintaining a vast range of mammalian cell types