Cytokine reagent FLT3LG

lyophilized

Fms-related tyrosine kinase 3 ligand (Flt3L); SL cytokine; Flt3 ligand; Description - Recombinant Mouse Fms-like Tyrosine Kinase 3 Ligand is produced by our Mammalian expression system and the target gene encoding Gly27-Arg188 is expressed with a 6His tag at the C-terminus. Calculated Molecular Weight - Mol Mass:19.4 KDa; APMol Mass:22-30 KDa, reducing conditions Formulation - Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Endotoxin - < 1 EU/µg as determined by LAL test Biological Activity - Loaded Recombinant Human FLT3 (C-Fc)on Pro-A Biosensor, can bind Recombinant Mouse FLT3LG (C-6His) with an affinity constant of 0.447 nM as determined in BLI assay. (Regularly tested) Reconstitution - Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. Shipping - The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below. Background - Fms-related tyrosine kinase 3 ligand(Flt3L) is a single-pass type I membrane protein and consists of 232 amino acids. Flt3L is a hematopoietic four helical bundle cytokine, structurally homologous to stem cell factor and colony stimulating factor. Flt3L synergizes well with a number of other colony stimulating factors and interleukins. Flt3L stimulates the proliferation and differentiation of various blood cell progentiors by activating FLT3.