Cytokine reagent RP0085

solutionlyophilizedprotein

Recombinant Human Interleukin-18 is produced by our E.coli expression system and the target gene encoding Tyr37-Asp193 is expressed. Calculated Molecular Weight - Mol Mass:18.2 KDa; APMol Mass:17 KDa, reducing conditions Endotoxin - < 1 EU/µg as determined by LAL test. Reconstitution - Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below. Background - Interleukin-18 is a secreted protein and it belongs to the IL-1 family. IL-18 is a proinflammatory cytokine and produced by macrophages and other cells. This cytokine can induce the IFN-gamma production of T cells. The combination of this cytokine and IL12 has been shown to inhibit IL-4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity. After stimulation with IL-18, natural killer (NK) cells and certain T cells release another important cytokine called interferon-γ (IFN-γ) or type II interferon that plays an important role in activating the macrophages or other cells.