Cytokine reagent RP0086

solutionlyophilizedprotein

Recombinant Mouse Interleukin-13 is produced by our Mammalian expression system and the target gene encoding Pro22-Phe131 is expressed with a 6His tag at the C-terminus. Calculated Molecular Weight - Mol Mass:13.1 KDa; APMol Mass:14-30 KDa, reducing conditions Endotoxin - < 1 EU/µg as determined by LAL test. Biological Activity - Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is 11.34 ng/ml. (Regularly tested) Reconstitution - Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. Shipping - The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below. Background - Mouse interleukin 13 (mIL-13) is a pleiotropic cytokine produced by activated Th2 cells. IL-13 induces B cell proliferation and immunoglobin production. It contains a four helical bundle with two internal disulfide bonds. Mouse IL13 shares 58% sequence identity with human protein and exhibits cross-species activity. IL13 signals via receptor IL13R (type2, IL4R) and activates STAT-6. IL13 initially binds IL-13Rα1 with low affinity and triggers association of IL4Rα, generating a high affinity heterodimeric receptor IL13R and eliciting downstream signals. IL13 also binds IL-13Rα2 with high affinity, which plays a role in a negative feedback system of IL13 signaling. IL13 is an important mediator of allergic inflammation and disease.