Cytokine reagent OSM

solutionlyophilizedprotein

Recombinant Human Oncostatin M is produced by our E.coli expression system and the target gene encoding Ala26-Arg221 is expressed with a 6His tag at the N-terminus. Calculated Molecular Weight - Mol Mass:24.44 KDa; APMol Mass:28 KDa, reducing conditions Formulation - Lyophilized from a 0.2 μm filtered solution of 20mMTris-HCl, 1mM EDTA, 200mM NaCl, pH 7.5. Endotoxin - < 0.01 EU/µg as determined by LAL test. Activity - Measured by the dose-dependent stimulation of TF-1 cells. The ED50 for this effect is 0.2-1 ng/ml. (QC verified) Reconstitution - Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. Shipping - The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below. Background - Oncostatin M (OSM) is a glycoprotein belonging to the interleukin-6 family of cytokines that includes leukemia-inhibitory factor, granulocyte colony-stimulating factor, and interleukin 6. OSM encodes a growth regulator, which Inhibits the proliferation of a number of tumor cell lines. It stimulates proliferation of AIDS-KS cells. OSM regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells. OSM is considered as a pleiotropic cytokine that initiates its biological activities through specific cell surface receptors.