Process laboratory biosensor Octet® AHC2

Process laboratory biosensor - Octet® AHC2 - Sartorius Group
Process laboratory biosensor - Octet® AHC2 - Sartorius Group
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Characteristics

Applications
process

Description

Rapid quantitation and kinetic analysis of crude and purified human Fc-region containing proteins Improved biosensor surface ligand loading capacity for enhanced kinetics assays sensitivity and increased dynamic range for quantitation assays Efficient and cost-effective regeneration for biosensor re-use for both kinetics and quantitation applications Direct quantitation of human IgGs in the range of 0.1 – 2,000 ug/mL The Octet® AHC2 Biosensors are the 2nd generation of anti-human IgG Fc capture biosensors that offer significantly improved performance as well as increased versatility by enabling both quantitation and kinetic characterization of human IgG antibodies and IgG-derived Fc-fusion proteins. Furthermore, cost-effective 10-20 times regeneration of the Octet® AHC2 Biosensors using a standard low pH protocol, makes them an extremely useful solution for a wide range of high-throughput applications, including lead identification and optimization, cell line screening, process development and QC. Range of Applications The Octet® AHC2 Biosensors offer a flexible platform for a broad range of applications from profiling the kinetics (kon, koff, and KD) or performing epitope binning between human Fc-containing proteins and their analytes to quantitation of human Fc-containing proteins in both purified and crude protein samples (e.g. supernatants and sera).

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