Laboratory biosensor Octet® ProL

laboratory biosensor
laboratory biosensor
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Description

Broader recognition of Ig isotypes and species than Protein A or G Regenerable and cost-effective format Binds whole molecule Ab and Fab fragments Quantitate in crude matrices Octet® ProL biosensors are coated with Protein L and provide a rapid and direct method for quantitating a broad set of kappa light chain containing immunoglobulins, including whole molecules, Fab fragments and single chain variable fragments, from buffer, conditioned media or complex matrices. The recombinant Protein L, which is factory-immobilized onto the biosensor, recognizes a wider range of antibody classes than both Protein A and Protein G, including IgG, IgM, IgA, IgE and IgD. The Octet® ProL biosensors are especially useful for quantifying antibodies and antibody fragments from serum based culture because Protein L does not bind bovine immunoglobulins, which often contaminate serum supplements. Note that Protein L does not recognize all kappa light chains. For example, Protein L recognizes mouse VKl (but not VKll, VKIII and VKIV) and human VKI, VKIII and VKIV (but not VKII).

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