Laboratory biosensor Octet® HIS1K

High specificity and affinity to a six-histidine or penta-histidine peptide tag (His-tag) Designed for use in buffer, complex media, column eluates, and cell lysates Binds His-tagged proteins even in the presence of imidazole Direct capture of His-tagged proteins for kinetic analysis with interacting protein analyte Easy to use, label-free, no wash protocol The polyhistidine protein tag (His-tag), common ly engineered into proteins during cloning, can be used to facilitate recombinant protein purification and detection by exploiting the chelating properties of histidine to an immobilized metal. The Octet® HIS1K Biosensors consist of high affinity, high specificity Penta-His antibody pre-immobilized on a fiber optic biosensor. In conjunction with the Octet® systems, the Octet® HIS1K Biosensors provide a rapid and label-free method for His-tagged protein quantitation and kinetic characterization. The high specificity of the antibody-based biosensor enables the direct capture and quantitation of His-tagged proteins even in crude cell lysates or supernatants.