Solution reagent kit HOT FIREPol® Multiplex qPCR Mix

DNA polymeraseTaq DNA polymerasediagnostic

Probe-based qPCR master mix that has been optimized for highly sensitive and accurate quantification of up to 4 targets in a single reaction. This master mix was developed for TaqMan® probes but is also suitable for other hydrolysis probe types. Analyze 1-4 targets in 1 reaction. High specificity and sensitivity. Robust amplification of GC-rich targets. Contains dUTP to prevent cross-contamination when. Used in combination with UNG. Description HOT FIREPol® Multiplex qPCR Mix is optimized for amplifying multiple targets in a single reaction in real-time quantitative PCR assays. The qPCR Mix comprises all the components necessary (except primers, probes, and template) to perform qPCR: HOT FIREPol® DNA Polymerase, optimized buffer components, ultrapure dNTPs, and MgCl2. HOT FIREPol® Multiplex qPCR Mix is optimized for DNA hydrolysis probes based on the 5' flap endonuclease activity. HOT FIREPol® DNA Polymerase is activated by a 10 min incubation step at 95°C. This prevents the extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup. Properties Concentration: 5x Hot-start: yes, initial activation in 10 min Detection type: Probe-based Reference dye: ROX and Purple dye options are available. Also possible to have no dye. Suitable qPCR Cyclers: all cyclers that do not need passive reference dye. Mix Components HOT FIREPol® DNA Polymerase 5x Multiplex qPCR buffer with 15 mM MgCl2 dNTPs including dUTP