Solution reagent kit HOT FIREPol® Probe Mix Plus (without ROX)
DNA polymeraseTaq DNA polymerasefor microbiology

solution reagent kit
solution reagent kit
solution reagent kit
Add to favorites
Compare this product
 

Characteristics

Type
solution, DNA polymerase, Taq DNA polymerase
Applications
for genotyping, diagnostic, for research, laboratory, for nucleic acids, for environmental analysis, for qPCR, for DNA analysis, for DNA quantification, for microbiology, for forensics, multi-function, for amplification reaction, for DNA amplification
Format
liquid
Tested parameter
for DNA
Other characteristics
hot start
Storage temperature

-20 °C
(-4 °F)

Description

Sensitive qPCR Mix designed for effective quantification of DNA targets with probe-based chemistry. Cost-effective real-time qPCR master mix for probe-based qPCR assays. This master mix has been developed for TaqMan® probes but is suitable for other hydrolysis probe types as well. Suitable for singleplex and duplex assays. High specificity and sensitivity. Reaction set-up and shipment without dry ice. Description HOT FIREPol® Probe qPCR Mix Plus (no ROX) is optimized for real-time quantitative PCR assays and contains all the components necessary to perform qPCR, with the exception of template, primers, and probe. The qPCR Mix contains optimized components and HOT FIREPol® DNA Polymerase supplied in a proprietary reaction buffer that enables detection of low copy number targets. HOT FIREPol® Probe qPCR Mix Plus (no ROX) is optimized for DNA hydrolysis probes based on the 5´ flap endonuclease activity. HOT FIREPol® DNA Polymerase is activated by a 12 min incubation step at 95°C. This prevents the extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup. Properties Concentration: 5x Hot-start: yes, initial activation in 12-15 min Detection type: Probe-based Reference dye: none Compatible real-time instruments: cyclers that do not need ROX Mix Components HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabeling hot-start. 5x Probe qPCR buffer with 15 mM MgCl2: 1x PCR solution – 3 mM MgCl2. dNTPs: dATP, dCTP, dGTP and dTTP. Some applications of this product may require a license which is not provided by the purchase of this product.

Catalogs

No catalogs are available for this product.

See all of Solis BioDyne‘s catalogs

Exhibitions

Meet this supplier at the following exhibition(s):

ANALYTICA 2024
ANALYTICA 2024

9-12 Apr 2024 München (Germany) Hall A3.210 - Stand Vide

  • More information

    Other Solis BioDyne products

    qPCR and RT-qPCR

    *Prices are pre-tax. They exclude delivery charges and customs duties and do not include additional charges for installation or activation options. Prices are indicative only and may vary by country, with changes to the cost of raw materials and exchange rates.