Solution reagent HOT FIREPol® SolisGreen® qPCR Mix

DNA polymerasedyeTaq DNA polymerase

Highly sensitive dye-based qPCR Mix with improved detection of low-copy targets. Optimized ready-to-use solution for dye-based real-time quantitative PCR assays. SolisGreen® dye is characterized by high sensitivity and great PCR efficiency with low template amounts for accurate and reproducible results. Improved detection of low target concentrations. Higher fluorescence level. Reaction set-up and shipment without dry ice. Description Dye-based real-time quantitative PCR (qPCR) uses DNA binding dye to evaluate the DNA amplification process during PCR. HOT FIREPol® SolisGreen® qPCR Mix is an optimized ready-to-use solution for real-time quantitative PCR assays, incorporating SolisGreen® dye. It comprises all the components necessary, excluding the template and primers, to perform highly sensitive qPCR. The user simply needs to add water, template, and primers. HOT FIREPol® DNA Polymerase is activated by a 10 min incubation step at 95°C. This prevents the extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup. Applications Detection and quantification of DNA and cDNA targets Profiling gene expression Microbial detection Viral load determination Properties Concentration: 5x Hot-start: yes, initial activation in 10 min Detection type: dye-based, includes SolisGreen® intercalating dye Reference dye: based on ROX Mix Components HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabling hot-start. 5x qPCR buffer with 12.5 mM MgCl2: 1x PCR solution – 2.5 mM MgCl2. dNTPs: dATP, dCTP, dGTP and dTTP. SolisGreen® dye. Internal reference based on ROX dye.