Enzyme reagent kit SOLIScript® 1-step SolisGreen®

solutiondyefor microbiology

Highly sensitive dye-based one-step RT-qPCR. SOLIScript® 1-step SolisGreen® Kit is an optimized solution for highly sensitive real-time one-step RT-PCR (RT-qPCR) using intercalating dye detection via the SYBR®/FAM fluorescence channel. cDNA synthesis and dye-based qPCR in one tube. High specificity, sensitivity and yield. Reaction set-up and shippment without ice. Consistent results with low RNA input. RNase inhibitor included in kit. Compatible with most cyclers. Description SOLIScript® 1-step SolisGreen® Kit is optimized for highly sensitive and accurate quantification of RNA targets by real-time one-step RT-PCR (RT-qPCR) using dsDNA-intercalating dye detection via the SYBR®/FAM fluorescence channel. Compatible with ROX-independent and ROX-dependent qPCR cyclers (excluding high-ROX platforms). The Kit comes in 3 tubes (including water) and contains all the components necessary (except RNA template and primers) to perform cDNA synthesis and qPCR in a single tube. Properties Sample type: RNA Product format: Reagent kit No of tubes: three tubes Detection type: Intercalating dye-based Reference dye: reference dye based on ROX Compatible qPCR cyclers: The Kit is compatible with ROX-independent and ROX-dependent qPCR platforms (excluding high-ROX machines, such as Applied Biosystems™ 5700, 7000, 7700, 7900HT, StepOne™, StepOnePlus™). Kit Components 40x One-step SOLIScript® Mix: SOLIScript® Reverse Transcriptase, RiboGrip™ RNase Inhibitor 5x One-step SolisGreen® Mix: HOT FIREPol® DNA polymerase, dNTPs (dATP, dCTP, dGTP, dTTP), 12.5 mM MgCl2 (1x RT-qPCR solution – 2.5 mM MgCl2), SolisGreen® dsDNA-intercalating dye, passive reference dye based on ROX dye.