Stain reagent kit TB (Kinyoun)

bacteriaMycobacterium tuberculosis

A modified basic fuchsin stain (without heating) for the identification of acid-fast bacteria such as Mycobacterium tuberculosis. Principle: This staining kit is based on the Ziehl-Neelsen stain (a carbofulfuchsin solution) recommended by the WHO. Acid-fast bacteria such as Mycobacterium tuberculosis and Mycobacterium leprae are difficult to stain because of their lipoid capsule in the cell wall. However, once the lipoid capsule is stained with an enhanced dye such as carbolfuchsin, the newly formed compound will resist decolorization from acid-alcohol and retain the original color stained (red), which can be easily differentiated from other microorganisms that still adsorb the counter blue stain. Methods: Cold stain: 1. Fix smear with flame and then add Carbolfuchsin to stain for 5 minutes or longer (without heating). 2. Rinse with water gently (Blot up the slide gently with bibulous paper) 3. Decolorize for 2 minutes with Acid Alcohol. 4. Rinse with water gently (if red color is still visible on the surface of specimen, add Acid Alcohol again until the red color is completely decolorized). 5. Apply Methylene blue for 30 seconds. Rinse with water gently and air dry. Examine the finished slide under a microscope. Precaution: 1. This method is also used for heating stain. 2. When decolorizer is used up, we recommend that users should buy the solution from Baso. 3. After each use, cap the reagent bottle immediately to avoid vaporization. 4. For sputum specimen, the smear should be properly thickened to increase detection rate. For thicker smear, the counterstain time must be controlled. The microscopic examination will be affected if background is too dark.