Stain reagent Metachromatic Granules Stain

for microbiologybacteria

Principle: The size of Corynebacterium diphtheriae is very variable, essentially rod-shaped but often showing irregular expansion at one end. Cells frequently lie in small clusters at acute angles to each other in V, L or Y shapes. Gram positive but readily decolorized, non-motile, non-capsulate, non-sporing. Strains are distributed as beans and stained unevenly. With Metachromatic Granules Stain, deeply stained granules known as metachromatic granules, are found on one side or both sides of diphtheriae. Specifications: Contents: 2 vials × 20 ml/Kit Components: - Solution A: 1 vial × 20 ml, Toluidine Blue - Solution B: 1 vial × 20 ml, Potassium iodide Methods: 1. Fix smears on flames, add solution A to stain for 3~5 minutes and rinse with water. 2. Add solution B to stain for 1 minute and rinse with water. 3. Dry and observe microscopically. - Product: Metachromatic Granules Stain - Application: Microbiology, staining of Corynebacterium diphtheriae - Kit contents: 2 vials × 20 ml (Solution A: Toluidine Blue, Solution B: Potassium iodide) - Release time: 2016-8-12 - Non-motile, non-capsulate, non-sporing, Gram positive (readily decolorized)