LINEAR CHEMICALS chromogenic reagents

Storage temperature: 2 °C - 8 °C

TOTAL and PROSTATIC Colorimetric method KINETIC The method is based on the hydrolysis of α-naphtyl phosphate at pH 5.0 by acid phosphatase (ACP) to produce α-naphtol and inorganic phosphate. The pentanediol acts ...

Storage temperature: 2 °C - 8 °C

Colorimetric method ENDPOINT PRINCIPLE The method is based on the specific binding of bromocresol green (BCG), an anionic dye, and the protein at acid pH with the resulting shift in the absorption wavelength of the ...

Storage temperature: 2 °C - 8 °C

DGKC Colorimetric method KINETIC PRINCIPLE Alkaline phosphatase (ALP) catalyze the hydrolysis of 4-nitrophenylphosphate (4-NPP) with the formation of free 4-nitrophenol and inorganic phosphate, acting the alkaline ...

Colorimetric enzymatic method KINETIC PRINCIPLE In this direct method α-amylase catalyzes the hydrolysis of 2-chloro-p-nitrophenyl-α-D-maltotrioside (CNP-G3) substrate at pH 6.0 forming 2-chloro-p-nitrophenol ...

Storage temperature: 2 °C - 8 °C

TOTAL and DIRECT Colorimetric method END POINT PRINCIPLE Bilirubin is converted to coloured azobilirubin by diazotized sulfanilic acid and is measured photometrically. Of the two bilirubin fractions in serum –bilirubin-glucuronide ...

Storage temperature: 2 °C - 8 °C

DIRECT DPD – Colorimetric method END POINT WITH SAMPLE BLANK PRINCIPLE Direct bilirubin (conjugated) reacts with the diazonium salt 2,4-dichlorophenyldiazonium (2,4-DPD) in the presence of sulfamic acid to form ...

Storage temperature: 2 °C - 8 °C

TOTAL Colorimetric method ENDPOINT PRINCIPLE The method is based on the specific binding of arsenazo III and calcium at acid pH with the resulting shift in the absorbtion wavelength of the complex. The intensity ...

Storage temperature: 2 °C - 8 °C

THIOCYANATE Colorimetric method ENDPOINT PRINCIPLE Chloride ions in the sample quantitatively displaces thiocyanate from mercuric thiocyanate. Liberated thiocyanate ion reacts with ferric ion forming a red ferric-thiocyanate ...

Storage temperature: 2 °C - 8 °C

TOTAL Enzymatic colorimetric method ENDPOINT PRINCIPLE This method for the measurement of total cholesterol in serum involves the use of three enzymes: cholesterol esterase (CE), cholesterol oxidase (CO) and peroxidase ...

Storage temperature: 2 °C - 8 °C

TOTAL AND INHIBITED Enzymatic colorimetric method KINETIC PRINCIPLE Cholinesterase (CHE) catalyzes the hydrolysis of butyrylthiocholine substrate forming butyrate and thiocholine. The latter reduces 5,5´- mercaptobis-2-nitrobenzoic ...