Lyme disease test kit RealLine

Borrelia burgdorferiBorrelia gariniiBorrelia afzelii

Description
The RealLine Borrelia burgdorferi s.l. kits detect the B. burgdorferi sensu lato group (Borrelia afzelii, Borrelia garinii, Borrelia burgdorferi sensu stricto) DNA from ticks and human samples by Real-Time PCR. All components are lyophilized and ready-to-use. GLU – for General Lab Use.

Clinical background
Lyme disease (borreliosis) is caused by Gram-negative spirochetes of the genus Borrelia, collectively referred to as the Borrelia burgdorferi sensu lato group. Timely molecular detection supports diagnosis and epidemiological surveillance.

Sample Material

• Blood serum, plasma
• Cerebrospinal fluid
• Tick suspension samples

Detection Channels

• FAM (495 – 520 nm) for Internal Control (IC)
• ROX (575 – 602 nm) for Borrelia burgdorferi s.l. DNA

Formats
Str-format: In Str-format kits the Ready-Mastermix is lyophilized and prepared in 0.2 ml white tubes arranged in a plate. For preparation the required number of tubes can be cut. 50 µl of extracted DNA/RNA are pipetted into the tubes, close with PCR foil or caps and run the assay. Tubes are medium-profile and compatible with RealLine Cyclers, BIO-RAD cyclers and others.

Validated Cyclers

• RealLine Cycler 48 or RealLine Cycler 96 (BIORON GmbH)
• CFX96, iQ5 iCycler (Bio-Rad, USA)
• DT96, DTLite (DNA-Technology, Russia)

Additional information
Available variation example: 48 Tests. For optimal results use the RealLine Internal Control (REF VBC8881-R) where applicable. Read the Instructions for Use (IFU) carefully.

Characteristics / technical specifications

• Denomination: RealLine Borrelia burgdorferi s.l.
• REF / SKU: VBD1498-R
• Available variation: 48 Tests
• Components: lyophilized, ready-to-use Ready-Mastermix
• Intended use: detection of B. burgdorferi sensu lato DNA by Real-Time PCR (GLU)
• Sample types: blood serum, plasma, cerebrospinal fluid, tick suspensions
• Detection channels: FAM (IC), ROX (Borrelia DNA)
• Wavelengths: FAM 495 – 520 nm; ROX 575 – 602 nm
• Format: Str-format (0.2 ml tubes, medium-profile)
• Validated cyclers: RealLine Cycler 48/96, Bio-Rad CFX96/iQ5, DT96/DTLite