Monoclonal antibody MTAE913

chromatographyfor ELISAimmunoglobulin

This monoclonal antibody enables specific detection of Syrian hamster IgG in ELISA. Recommendation MTAE913 is recommended as detection mAb in ELISA in combination with capture mAb MTAE142 (product code 3154-3). Product details Conjugate - Biotin Immunogen - Native Syrian hamster IgG (Mesocricetus auratus, golden hamster) Host - Mouse Reactivity - Hamster Cross-reactivity - No cross-reactivity with IgG or IgM from Armenian hamster or with IgG from cow, sheep, goat, horse, pig, dog, cat, ferret, guinea pig, rabbit, rat, mouse, macaques, or human. Purification - Purified from in vitro cultures by protein G affinity chromatography. Biotinylation - Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin. Concentration - 0.5 mg/ml Supplied in - PBS with 0.02% sodium azide. Sterile-filtered (0.2 µm). Shipping - Shipped at ambient temperature. Shelf life - At least 18 months from date of receipt. Analyte description Immunoglobulin G (IgG) is the most abundant Ig isotype in serum, making up approximately 80% of all serum immunoglobulins. In humans, there are four subclasses of immunoglobulin G, with the highest serum concentrations of IgG1 followed by IgG2, IgG3, and IgG4. In mice, the IgG subclasses are defined as IgG1, IgG2a/c, IgG2b, and IgG3. The IgG molecule consists of two heavy and two light chains (κ or λ), resulting in a molecule with two arms for antigen binding. High levels of IgG antibodies are induced following the initial IgM response in a typical immune response to antigens.