Solution reagent MT21

monoclonal antibodychromatographyfor research

This monoclonal antibody enables specific detection of human IL-21 in ELISA. Recommendation To retain detectable levels of IL-21 in cell culture supernatants, biotinylated mAb MT21.3m can be included during cell culture. The antibody will bind to secreted IL-21 thus preventing IL-21 from interacting with its receptor. In this way, IL-21 is kept in solution and can be quantified in ELISA. For this purpose, MT21.3m is free of preservatives. Please note that mAb MT21.3m is optimized for use with human IL-21 ELISA kits (product 3540-1A and 3540-1H) and anti-human IL-21 mAbs from Mabtech. Product details Product - Anti-human IL-21 mAb (MT21.3m) azide free, biotin Analyte - IL-21 Conjugate - Biotin Immunogen - Recombinant human IL-21 Host - Mouse Reactivity - Human, Non-human primates Specificity - Native and recombinant human IL-21 Cross-reactivity - Cross-reacts with IL-21 from cynomolgus macaques. For more information please check the Cross-reactivity guide section below. Purification - Purified from in vitro cultures by protein G affinity chromatography. Biotinylation - Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin. Concentration - 0.5 mg/ml Supplied in - PBS, free of preservatives. Sterile-filtered (0.2 µm). Contents - Monoclonal antibody MT21.3m, biotinylated. Supplied at 0.5 mg/ml in PBS, free of preservatives. Analyte description Interleukin 21 (IL-21) is primarily secreted by CD4 T cells (particularly follicular T cells and Th17) and is an important regulator of immune responses. For Th17 cells, IL-21 is an autocrine regulator sustaining Th17 development.