Research test kit AMPLICON™ HS

oncologymolecularfor single cell DNA sequencing

Swift Amplicon HS Panels enable ultra-low frequency variant detection at and below 1% MAF from 10-20 ng of cell-free DNA (cfDNA) for Illumina® sequencers. Nucleotide errors, normally introduced during PCR and sequencing, are removed through the implementation of unique molecular identifiers (MIDs or UMIs) that uniquely label target templates during amplification. Highlights • Sensitive and Specific robustly detect SNVs and indels down to 0.25% allele frequency • Compatible with cfDNA and FFPE, amplifies from 10-50 ng of cfDNA • Fast workflow, high quality data From DNA to Illumina® compatible libraries within 3 hours • Paired Data Analysis Tools-Cloud-based or open source Swift Amplicon HS Panels enable variant calling at and below 1% frequency, with allele sensitivity dependent on the amount of input material available and the depth of sequencing. Using 10 ng of input DNA, this assay consistently detects variants at 0.5% with a recommended sequencing depth of at least 60,000x (1M reads) prior to deduplication with MIDs and 0.25% from 20 ng of input DNA. Swift Amplicon HS Panels facilitate targeted variant calling of clinically relevant mutations at and below 1% frequency. The technology enables this by the incorporation of molecular identifiers (MIDs or UMIs), which improve sensitivity and specificity by removing false positive errors introduced during PCR and sequencing. Supported Applications • Oncology – liquid biopsy cfDNA and FFPE tissue • Genome/Host Detection – Graft vs. Host Disease and Fetal in Maternal cfDNA* • Custom targets* Specifications Input DNA Required and Limit of Detection 10 ng for 0.5% detection; 20 ng for 0.25% detection