Pregnancy test SaberVet PAG

for cattleveterinarywhole blood

Intended Use
The SaberVet Bovine Pregnancy Rapid Test is a qualitative lateral-flow immunoassay for detection of pregnancy-associated glycoproteins (PAGs) in peripheral blood of ruminants. It uses a double-antibody sandwich format on a nitrocellulose membrane. A visible colored band appears on the test line for positive samples. The test can be used from day 26 post-breeding and throughout pregnancy to support herd reproductive management.

Reagent and Materials Provided

• Test devices (sealed foil pouches)
• Assay buffer
• Disposable droppers
• Package insert / instructions

Materials Required but Not Provided

• Timer

Storage and Stability
Store sealed test devices at room temperature away from direct light (4–30°C). Do not freeze. Use before the expiration date printed on the pouch.

Warnings, Precautions and Safety Information

• For bovine use only; not for human diagnostics.
• Environmental conditions (temperature, humidity) can affect results; perform test within recommended conditions.
• Inspect foil pouch integrity before use and run the test immediately after opening.
• Do not reuse test devices or components.
• Do not use after the expiry date or if components from different lot numbers are mixed.
• All biological samples should be considered potentially infectious; wear protective gloves and follow local biosafety and disposal regulations.

Specimen Collection, Handling, and Transport

• Accepted sample types: whole blood (EDTA), plasma (EDTA, heparin or sodium citrate), or serum.
• Whole blood: collect and test promptly; if delay is necessary, refrigerate (2–8°C) and test within 24 hours.
• Serum: collect in non-anticoagulant tubes and centrifuge to separate serum.
• Plasma: collect in anticoagulant tubes and centrifuge to separate plasma.
• Short-term storage: 2–8°C; long-term storage: freeze at −20°C or below and avoid repeated freeze–thaw cycles.
• Clarify samples with precipitate prior to testing; hemolyzed or contaminated specimens may produce unreliable results.

Summary
Pregnancy-associated glycoproteins (PAGs) are secreted by embryonic trophoblast cells after conception and are present in peripheral blood of pregnant ruminants. The SaberVet rapid test provides a field-suitable method to detect PAGs in whole blood, plasma or serum from day 26 post-breeding, aiding veterinarians and livestock producers in reproductive decision-making, reducing unnecessary breeding intervals and optimizing herd productivity.

Features / Technical specifications

• Detection biomarker: Pregnancy-Associated Glycoproteins (PAGs)
• Sample types: whole blood (EDTA), plasma (EDTA/heparin/sodium citrate), serum
• Earliest detection: from 26 days post-breeding
• Test format: qualitative lateral-flow double-antibody sandwich on nitrocellulose
• Result indicator: colored (purple) test line for positive samples
• Reported sensitivity: 99%
• Reported specificity: 98.8%
• Shelf life: 24 months when stored under recommended conditions
• Storage conditions: 4–30°C; do not freeze; keep pouch sealed and protected from light
• Included components: test devices, buffer, disposable droppers, package insert
• Manufacturing & quality: manufactured in China in a CE, ISO 13485 & GMP certified facility; quality standards referenced: ISO 9001, GMP, CE, ISO 13485
• Intended users: veterinarians, dairy farms, beef production facilities