Medium reagent
for colorimetryfor amylaseenzymatic

medium reagent
medium reagent
medium reagent
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Characteristics

Type
reagent medium
Applications
for colorimetry
Tested parameter
for amylase
Method
enzymatic, colorimetric

Description

Colorimetric enzymatic method KINETIC PRINCIPLE In this direct method α-amylase catalyzes the hydrolysis of 2-chloro-p-nitrophenyl-α-D-maltotrioside (CNP-G3) substrate at pH 6.0 forming 2-chloro-p-nitrophenol (CNP) and free glycosides. The reaction is monitored kinetically at 405 nm by the rate of formation of the colored CNP produced, proportional to the activity of the α-amylase in the sample.

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CLINICAL CHEMISTRY

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