Solution reagent kit 1155005
diagnosticliquidtriglycerides

Solution reagent kit - 1155005 - LINEAR CHEMICALS - diagnostic / liquid / triglycerides
Solution reagent kit - 1155005 - LINEAR CHEMICALS - diagnostic / liquid / triglycerides
Solution reagent kit - 1155005 - LINEAR CHEMICALS - diagnostic / liquid / triglycerides - image - 2
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Characteristics

Type
solution
Applications
diagnostic
Format
liquid
Tested parameter
triglycerides
Method
enzymatic, colorimetric
Storage temperature

Max.: 8 °C
(46 °F)

Min.: 2 °C
(36 °F)

Description

The method is based on the enzymatic hydrolysis of serum or plasma triglyceride to glycerol and free fatty acids (FFA) by lipoprotein lipase (LPL). The glycerol is phosphorylated by adenosin triphosphate (ATP) in the presence of glycerolkinase (GK) to form glycerol-3-phosphate (G-3-P) and adenosine diphosphate (ADP). G-3-P is oxidized by glycerophosphate oxidase (GPO) to form dihydroxyacetone phosphate (DHAP) and hydrogen peroxide. A red chromogen is produced by the peroxidase (POD) catalyzed coupling of 4-aminoantipyrine (4-AA) and phenol with hydrogen peroxide (H2O2), proportional to the concentration of triglyceride in the sample.

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