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Solution reagent kit KR10150
diagnosticliquidhydrogen peroxide

Solution reagent kit - KR10150 - LINEAR CHEMICALS - diagnostic / liquid / hydrogen peroxide

Solution reagent kit - KR10150 - LINEAR CHEMICALS - diagnostic / liquid / hydrogen peroxide - image - 2

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Characteristics

Type

solution

Applications

diagnostic

Format

liquid

Tested parameter

hydrogen peroxide, creatinine

Method

enzymatic, colorimetric

Storage temperature

Max.: 8 °C
(46 °F)

Min.: 2 °C
(36 °F)

Description

This procedure is based on an improved enzymatic method originally designed to assess serum and urinary creatinine. The assay is performed in two stages. In the first stage, creatine is eliminated during the initial minutes of sample pre-incubation with creatinase. In the second stage, the addition of creatininase initiates the reaction, hydrolyzing creatinine in the sample in the presence of sarcosine oxidase (Sar OD) with the production of hydrogen peroxide:_x000D_ Creatinine + H2O → Creatine_x000D_ Sarcosine + H2O + O2 → H2O2 + Glycine + HCHO_x000D_ The hydrogen peroxide derived from the oxidase reaction is quantified by a Trinder-type reaction in which the chromogenic derivative HTIB and 4-aminoantipyrine (4-AA) condense in the presence of peroxidase (POD) to form a red quinonimine dye._x000D_ 4-AA + HTIB → Quinonimine + H2O_x000D_ The color development rate is proportional to the creatinine concentration in the sample.

Catalogs

General Catalogue

36 Pages

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