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Solution reagent kit Urea Bun BR
diagnosticliquidurea

solution reagent kit

solution reagent kit

solution reagent kit

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Characteristics

Type

solution

Applications

diagnostic

Format

liquid

Tested parameter

urea

Method

enzymatic

Storage temperature

Max.: 8 °C
(46 °F)

Min.: 2 °C
(36 °F)

Description

Urease/GlDH UV enzymatic method KINETIC PRINCIPLE Urea is hydrolyzed by urease to ammonia and carbon dioxide. The ammonia is converted to glutamate by glutamate dehydrogenase (GlDH) in the presence of NADH and oxoglutarate. The reaction is monitored kinetically at 340 nm by the rate of decrease in absórbanse resulting from the oxidation of NADH to NAD+, proportional to the concentration of urea present in the sample.

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