Solution reagent kit BERTHELOT
diagnosticliquidurea

solution reagent kit
solution reagent kit
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Characteristics

Type
solution
Applications
diagnostic
Format
liquid
Tested parameter
urea
Method
enzymatic, colorimetric
Storage temperature

Max.: 8 °C
(46 °F)

Min.: 2 °C
(36 °F)

Description

Urease/Salycilate Enzymatic colorimetric method ENDPOINT PRINCIPLE Urea is hydrolyzed by urease into ammonia and carbon dioxide. The ammonia generated reacts with alkaline hypochlorite and sodium salicylate in presence of sodium nitroprusside as coupling agent to yield a green cromophore. The intensity of the color formed is proportional to the concentration of urea in the sample.

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