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Solution reagent kit GOT/GPT color
diagnosticliquidALT

solution reagent kit

solution reagent kit

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Characteristics

Type

solution

Applications

diagnostic

Format

liquid

Tested parameter

ALT, for AST, for enzymes

Method

colorimetric

Storage temperature

Max.: 8 °C
(46 °F)

Min.: 2 °C
(36 °F)

Description

REITMAN-FRANKEL Colorimetric method ENDPOINT PRINCIPLE Aspartate aminotransferase (GOT) catalyzes the transfer of the amino group from aspartate to oxoglutarate with the formation of glutamate and oxalacetate. L-Aspartate + 2-Oxoglutarate L-Glutamate + Oxalacetate Alanine aminotransferase (GPT) catalyzes the transfer of the amino group from alanine to oxoglutarate with the formation of glutamate and pyruvate. L-Alanine + 2-Oxoglutarate Glutamate + Pyruvate The transaminase activity is proportional to the amount of oxalacetate or pyruvate formed over a definite period of time and is measured by the reaction with 2,4-dinitrophenylhydrazine (DNPH) and measurement of the color formed in an alkaline solution.

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