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Solution reagent kit
diagnosticliquidfor cholinesterase

solution reagent kit

solution reagent kit

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Characteristics

Type

solution

Applications

diagnostic

Format

liquid

Tested parameter

for cholinesterase

Method

enzymatic, colorimetric

Storage temperature

Min.: 2 °C
(36 °F)

Max.: 8 °C
(46 °F)

Description

TOTAL AND INHIBITED Enzymatic colorimetric method KINETIC PRINCIPLE Cholinesterase (CHE) catalyzes the hydrolysis of butyrylthiocholine substrate forming butyrate and thiocholine. The latter reduces 5,5´- mercaptobis-2-nitrobenzoic acid (DMNB) to 5-mercapto-2- nitrobenzoate (5-MNBA), a colored compound. The reaction is monitored kinetically at 405 nm by the rate of formation of the yellow color produced, proportional to the activity of CHE in the sample.

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