A Simple, Non-Radioactive Assay to Measure Glucose Uptake Inside Cells
Achieve sensitivity with broad linearity
Reliable and reproducible results
Glucose metabolism is a key process in many organisms. A lack of insulin-stimulated glucose uptake is associated with type 2 diabetes, while high glucose uptake is a sign of the high glycolytic rates associated with cancer. Measuring glucose uptake can determine the effects of various treatments for diabetes and cancer.
The gold standard method of assaying glucose uptake relies on detection of radio-labelled glucose analogs. Alternative colorimetric and fluorometric glucose uptake assays often lack the sensitivity and robustness needed to reliably measure glucose uptake in cells.
A Simple, Non-Radioactive Glucose Uptake Assay
The Glucose Uptake-Glo™ Assay is a plate-based, homogeneous bioluminescent method for measuring glucose uptake in cells, based on the detection of 2-deoxyglucose-6-phosphate (2DG6P).
Simple, homogeneous protocol: After addition of 2DG, there are no wash steps—all steps are additions.
Sensitive with broad linearity: The Glucose Uptake-Glo™ Assay can detect 0.5–30µM 2DG6P, and generates a signal-to-background > 3 with as few as 5,000 cells.
Compatible with automation: The “add-mix-measure” format is compatible with automated and high-throughput workflow; reactions are scalable for use in 96- and 384-well plates.
Reliable and reproducible: The Glucose Uptake-Glo™ Assay yields Z factors > 0.5