A Simple, Non-Radioactive Assay to Measure Glucose Uptake Inside Cells
Homogeneous protocol
Achieve sensitivity with broad linearity
Reliable and reproducible results
Glucose metabolism is a key process in many organisms. A lack of insulin-stimulated glucose uptake is associated with type 2 diabetes, while high glucose uptake is a sign of the high glycolytic rates associated with cancer. Measuring glucose uptake can determine the effects of various treatments for diabetes and cancer.
The gold standard method of assaying glucose uptake relies on detection of radio-labelled glucose analogs. Alternative colorimetric and fluorometric glucose uptake assays often lack the sensitivity and robustness needed to reliably measure glucose uptake in cells.
A Simple, Non-Radioactive Glucose Uptake Assay
The Glucose Uptake-Glo™ Assay is a plate-based, homogeneous bioluminescent method for measuring glucose uptake in cells, based on the detection of 2-deoxyglucose-6-phosphate (2DG6P).