Medical research assay kit ROS-Glo™

for oxidative stresshydrogen peroxidecell

Fast assay involves a simple two-reagent-addition protocol Substrate reacts directly with H2O2; no need for HRP as a coupling enzyme Overview The ROS-Glo™ H₂O₂ Assay is a homogeneous, fast and sensitive bioluminescent assay that measures the level of hydrogen peroxide (H₂O₂), a reactive oxygen species (ROS), directly in cell culture or in defined enzyme reactions. A derivatized luciferin substrate is incubated with sample and reacts directly with H₂O₂ to generate a luciferin precursor. Addition of ROS-Glo™ Detection Solution converts the precursor to luciferin and provides Ultra-Glo™ Recombinant Luciferase to produce light signal that is proportional to the level of H₂O₂ present in the sample. The H₂O₂ Substrate reacts directly with H₂O₂ to create the Luciferin Precursor. The ROS-Glo™ Substrate Detection Solution converts the Luciferin Precursor to luciferin and also provides the Ultra-Glo™ Luciferase to produce light. Light signal is proportional to the amount of H₂O₂ in the sample well. Overview of the ROS-Glo™ H₂O₂ Assay Protocol for Cell-based or Biochemical Detection The assay follows a simple two-reagent-addition protocol that does not require sample manipulation. The ROS-Glo™ H₂O₂ Substrate can be added to assay wells with the treatment or at the end of the treatment incubation period, and data can be recorded in less than 2 hours after reagent addition. Perform Enzymatic or Cell-based Assays in 96- and 384-well Plate Formats Menadione treatment of K562 cells resulted in a concentration-dependent ROS increase as detected with the ROS-Glo™ H₂O₂ Assay.